Biolegend, 354933, Alexa Fluor® 594 anti-human CD21 Antibody
25microg
Catalog Number: 354933
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4. |
| Application References(PubMed link indicates BioLegend citation) | Björck P, et al. 1993. Eur. J. Immunol. 23:1771. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC) |
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| RRID | AB_3105989 (BioLegend Cat. No. 354933) AB_3105989 (BioLegend Cat. No. 354934) |
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| Structure | Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD |
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| Distribution | Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells |
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| Function | Epstein-Barr virus receptor on B cells used for infection |
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| Interaction | CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1 |
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| Ligand/Receptor | C3d, EBV |
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| Cell Type | B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes |
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| Biology Area | Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc. 2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194. 3. Szakonyi G, et al. 2001. Science 292:1725. 4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881. |
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| Gene ID | 1380 |
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| UniProt | View information about CD21 on UniProt.org |
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| Clone | Bu32 |
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| Regulatory Status | RUO |
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| Workshop | V CD21.4, VI CD21.5 |
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| Other Names | Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor |
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| Isotype | Mouse IgG1, κ |
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