Biolegend, 356906, Alexa Fluor® 647 anti-human CD185 (CXCR5) Antibody
100tests
Catalog Number: 356906
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CXCR5-transfected cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Product Citations | Giles JR, et al. 2022. Immunity. 55:557. PubMed Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed |
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| RRID | AB_2561814 (BioLegend Cat. No. 356905) AB_2561815 (BioLegend Cat. No. 356906) |
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| Structure | G-protein coupled receptor, 7 transmembrane regions, 42 kD |
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| Distribution | Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells. |
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| Function | Mediates cell migration to the B cell follicles in the secondary lymphoid organs |
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| Ligand/Receptor | CXCL13 |
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| Cell Type | B cells, Tfh |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560. |
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| Gene ID | 643 |
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| UniProt | View information about CD185 on UniProt.org |
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| Clone | J252D4 |
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| Regulatory Status | RUO |
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| Other Names | BLR1, MDR15 |
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| Isotype | Mouse IgG1, κ |
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