Biolegend, 357317, Alexa Fluor® 647 anti-human Ganglioside GD2 Antibody
25tests
Catalog Number: 357317
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Neuroblastoma cell line LAN-1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a. |
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| Application References(PubMed link indicates BioLegend citation) | Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt) Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt) Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC) |
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| RRID | AB_2800980 (BioLegend Cat. No. 357317) AB_2800981 (BioLegend Cat. No. 357318) |
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| Distribution | Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma |
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| Function | Cell attachment to extracellular matrix |
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| Cell Type | Mesenchymal cells, Neurons |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | 1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260. 2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740. 3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200. |
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| Gene ID | 2583 |
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| UniProt | View information about Ganglioside GD2 on UniProt.org |
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| Clone | 14G2a |
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| Regulatory Status | RUO |
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| Other Names | Disialoganglioside GD2, Ganglioside G2 |
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| Isotype | Mouse IgG2a, κ |
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