Biolegend, 357422, Alexa Fluor® 647 anti-human CD4 Antibody
100tests
Catalog Number: 357422
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Human CD4 T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Product Citations | Remes Lenicov F, et al. 2018. Front Immunol. 9:1441. PubMed |
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| RRID | AB_2716180 (BioLegend Cat. No. 357421) AB_2716181 (BioLegend Cat. No. 357422) |
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| Structure | 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily |
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| Distribution | Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells |
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| Interaction | Part of the TCR/CD3 complex |
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| Ligand/Receptor | β2 domain from the MHC class II |
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| Cell Type | T cells, Monocytes, Macrophages, NKT cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557. |
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| Gene ID | 920 |
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| UniProt | View information about CD4 on UniProt.org |
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| Clone | A161A1 |
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| Regulatory Status | RUO |
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| Other Names | T4/Leu-3 |
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| Isotype | Rat IgG2b, κ |
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