Biolegend, 358911, Alexa Fluor® 647 anti-human CD199 (CCR9) Antibody
25tests
Catalog Number: 358911
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Cells transfected with human CCR9 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Product Citations | Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed |
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| RRID | AB_2562523 (BioLegend Cat. No. 358911) AB_2562524 (BioLegend Cat. No. 358912) |
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| Structure | A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart. |
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| Distribution | CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells |
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| Function | May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1 |
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| Ligand/Receptor | CCL25 |
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| Cell Type | T cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Zaballos A, et al. 1999. J. Immunol. 162:5671. 2. Wurbel MA, et al. 2007. J. Immunol. 178:7598. 3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73. |
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| Gene ID | 10803 |
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| UniProt | View information about CD199 on UniProt.org |
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| Clone | L053E8 |
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| Regulatory Status | RUO |
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| Other Names | GPR 9-6, GPR28, CDw199, Chemokine Receptor 9 |
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| Isotype | Mouse IgG2a, κ |
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