Biolegend, 359706, Alexa Fluor® 647 anti-human HIF1α Antibody
100tests
Catalog Number: 359706
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant HIF1α |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Product Citations | Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed Koers J, et al. 2023. Front Immunol. 13:1082154. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed |
|---|
| RRID | AB_2563331 (BioLegend Cat. No. 359705) AB_2563349 (BioLegend Cat. No. 359706) |
|---|
| Structure | 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains |
|---|
| Distribution | Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation |
|---|
| Function | Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations |
|---|
| Interaction | Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2 |
|---|
| Biology Area | Cell Biology, Immunology, Innate Immunity, Transcription Factors |
|---|
| Molecular Family | Nuclear Markers |
|---|
| Antigen References | 1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. 2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513. 3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230. 4. Galbraith MD, et al. 2013. Cell. 153:1327. |
|---|
| Gene ID | 3091 |
|---|
| UniProt | View information about HIF1alpha on UniProt.org |
|---|
| Clone | 546-16 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha |
|---|
| Isotype | Mouse IgG2b, κ |
|---|
