Biolegend, 359707, Alexa Fluor® 488 anti-human HIF1α Antibody
25tests
Catalog Number: 359707
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant HIF1α |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Blue Laser (488 nm) |
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| Product Citations | Wu XH, et al. 2023. Nat Commun. 14:1820. PubMed Kotzé LA, et al. 2021. mSphere. 6:e0055221. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed |
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| RRID | AB_2563975 (BioLegend Cat. No. 359707) AB_2563976 (BioLegend Cat. No. 359708) |
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| Structure | 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains |
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| Distribution | Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation |
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| Function | Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations |
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| Interaction | Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2 |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. 2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513. 3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230. 4. Galbraith MD, et al. 2013. Cell. 153:1327. |
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| Gene ID | 3091 |
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| UniProt | View information about HIF1alpha on UniProt.org |
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| Clone | 546-16 |
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| Regulatory Status | RUO |
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| Other Names | MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha |
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| Isotype | Mouse IgG2b, κ |
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