Biolegend, 360714, Alexa Fluor® 647 anti-human CD16 Antibody
100tests
Catalog Number: 360714
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | NK cell-enriched fraction from human peripheral blood. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84. Donor variability has been observed for clone B73.1 staining1, especially on granulocytes. |
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| Application References(PubMed link indicates BioLegend citation) | Perussia B, et al. 1983. J. Immunol. 130:2133. Lanier LL, et al. 1985. J. Exp. Med. 162:2089. Perussia B, et al. 1984. J. Immunol. 133:180. Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope) |
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| RRID | AB_2563020 (BioLegend Cat. No. 360713) AB_2563021 (BioLegend Cat. No. 360714) |
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| Structure | Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD) |
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| Distribution | NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells |
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| Function | Low affinity IgG Fc receptor, phagocytosis, ADCC |
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| Ligand/Receptor | IgG Fc receptor III (FcγRIII) |
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| Cell Type | Macrophages, Monocytes, Neutrophils, NK cells, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711. 2. Palmer BE, et al. 2005. J. Immunol. 175:8415. 3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183. 4. Wood KL, et al. 2005. Clin. Immunol. 117:294. 5. Björkström NK, et al. 2008. J. Immunol. 181:4219. |
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| Gene ID | 22142215 |
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| UniProt | View information about CD16 on UniProt.org |
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| Clone | B73.1 |
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| Regulatory Status | RUO |
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| Other Names | FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a |
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| Isotype | Mouse IgG1, κ |
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