Biolegend, 362607, Alexa Fluor® 488 anti-His Tag Antibody
25microg
Catalog Number: 362607
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Category
| Verified Reactivity | Human, Mouse, Rat, All species |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | KLH-conjugated His-tag peptide. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 2.0 μg/ml (1:250 - 1:2500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC. Clone J095G46 has been verified for immunocytochemistry (ICC). |
| Product Citations | Yao M, et al. 2023. JCI Insight. :8. PubMed |
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| RRID | AB_2734397 (BioLegend Cat. No. 362607) |
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| Structure | 6 repeat His amino acid |
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| Function | Epitope tag |
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| Biology Area | Cell Biology, Immunology |
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| Antigen References | Zhao C, et al. 2010. Anal Biochem. 399:237 Salichs E, et al. 2009. PLoS Genet. 5:e1000397 Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523 Lindner, P, et al. 1997. Biotechniques. 22:140 Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. |
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| Gene ID | NA |
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| UniProt | View information about His Tag on UniProt.org |
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| Clone | J095G46 |
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| Regulatory Status | RUO |
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| Other Names | Hexa histidine-tag, 6xHis-tag, 6XHis, 6His |
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| Isotype | Mouse IgG2a, κ |
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