Biolegend, 364105, Alexa Fluor® 488 anti-BrdU Antibody
25tests
Catalog Number: 364105
Send an RFQ.
Category
Loading…
| Antibody Type | Monoclonal |
|---|---|
| Host Species | Mouse |
| Immunogen | Iodouridine-conjugated ovalbumin |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality testedIHC - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. Hirota K, et al. 2007. J. Exp. Med. 204:41. Godebu E, et al. 2008. J. Immunol. 181:1798. Waskow C, et al. 2008. Nat. Immunol. 9:676. |
|---|
| Product Citations | Kaymak A, et al. 2018. Oncotarget. 9:28666. PubMed Berges C, et al. 2015. J Leukoc Biol. 98: 1091 - 1105. PubMed Bonnet C, et al. 2021. iScience. 24:103399. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Davenne T, et al. 2020. Cell Rep. 31:107640. PubMed Li H et al. 2019. Developmental cell. 49(1):118-129 . PubMed Li P, et al. 2022. EMBO Mol Med. 14:e14511. PubMed Radke J, et al. 2022. Nat Commun. 13:7304. PubMed |
|---|
| RRID | AB_2564499 (BioLegend Cat. No. 364105) AB_2564500 (BioLegend Cat. No. 364106) |
|---|
| Structure | Uridine derivative that can be incorporated into DNA and substitute thymidine residues. |
|---|
| Distribution | Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase. |
|---|
| Function | The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period. |
|---|
| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology |
|---|
| Molecular Family | Nuclear Markers |
|---|
| Antigen References | 1. Barker JM, et al. 2013. PLoS One 8:e63692. 2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205. 3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855. 4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954. 5. van der Wath RC, et al. 2009. PLoS One 4:e6972. 6. Dolbeare F, et al. 1985. Cytometry 6:521. 7. Gratzner HG. 1982. Science 218:474. |
|---|
| Gene ID | NA |
|---|
| Clone | 3D4 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | 5-bromodeoxyuridine, bromodeoxyuridine |
|---|
| Isotype | Mouse IgG1, κ |
|---|
