Biolegend, 364112, Alexa Fluor® 700 anti-BrdU Antibody
100tests
Catalog Number: 364112
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| Antibody Type | Monoclonal |
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| Host Species | Mouse |
| Immunogen | Iodouridine-conjugated ovalbumin |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy. |
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| Application References(PubMed link indicates BioLegend citation) | Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. Hirota K, et al. 2007. J. Exp. Med. 204:41. Godebu E, et al. 2008. J. Immunol. 181:1798. Waskow C, et al. 2008. Nat. Immunol. 9:676. |
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| RRID | AB_2814312 (BioLegend Cat. No. 364111) AB_2814313 (BioLegend Cat. No. 364112) |
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| Structure | Uridine derivative that can be incorporated into DNA and substitute thymidine residues. |
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| Distribution | Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase. |
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| Function | The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period. |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Barker JM, et al. 2013. PLoS One 8:e63692. 2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205. 3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855. 4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954. 5. van der Wath RC, et al. 2009. PLoS One 4:e6972. 6. Dolbeare F, et al. 1985. Cytometry 6:521. 7. Gratzner HG. 1982. Science 218:474. |
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| Gene ID | NA |
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| Clone | 3D4 |
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| Regulatory Status | RUO |
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| Other Names | 5-bromodeoxyuridine, bromodeoxyuridine |
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| Isotype | Mouse IgG1, κ |
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