Biolegend, 366312, Alexa Fluor® 647 anti-human CD18 Antibody
100tests
Catalog Number: 366312
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified human LFA-1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-11,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team). |
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| Application References(PubMed link indicates BioLegend citation) | Petruzzelli L, et al. 1995. J. Immunol. 155:854. (Activ)Lu C, et al. 2001. J. Immunol. 166:5629. (Activ) |
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| Product Citations | Cremasco F, et al. 2021. PLoS One. 16:e0241091. PubMed |
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| RRID | AB_2721410 (BioLegend Cat. No. 366311) AB_2721411 (BioLegend Cat. No. 366312) |
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| Structure | 90 - 95 kD type I transmembrane glycoprotein. |
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| Distribution | Leukocytes. |
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| Function | Cell adhesion and signaling. |
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| Interaction | Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c |
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| Ligand/Receptor | ICAMs. |
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| Cell Type | Leukocytes |
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| Biology Area | Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer TA. 1994. Cell 76:301. |
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| Gene ID | 3689 |
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| UniProt | View information about CD18 on UniProt.org |
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| Clone | CBR LFA-1/2 |
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| Regulatory Status | RUO |
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| Other Names | Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin |
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| Isotype | Mouse IgG1, κ |
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