Biolegend, 366921, Alexa Fluor® 488 anti-human IgG Fc Recombinant
25tests
Catalog Number: 366921
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| Verified Reactivity | Human |
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| Antibody Type | Recombinant |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Anti-human IgG Fc antibody (clone QA19A42) may cross-react with bovine IgG. Anti-human IgG Fc antibody (clone M1310G05) does not exhibit this cross-reactivity. |
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| RRID | AB_2890799 (BioLegend Cat. No. 366921) AB_2890799 (BioLegend Cat. No. 366922) |
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| Structure | Homodimer formed by the constant region of the IgG heavy chain |
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| Ligand/Receptor | CD16, CD32, CD64 |
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| Cell Type | B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets |
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| Biology Area | Adaptive Immunity, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | Fc Receptors |
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| Antigen References | Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA. |
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| Gene ID | 3500 |
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| UniProt | View information about IgG Fc on UniProt.org |
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| Clone | QA19A42 |
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| Regulatory Status | RUO |
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| Other Names | Immunoglobulin G |
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| Isotype | Mouse IgG1, κ |
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