Biolegend, 367127, Alexa Fluor® 647 anti-human CD14 Antibody
25tests
Catalog Number: 367127
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified human peripheral blood monocytes. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028. Devitt A, et al. 1998. Nature 6675:505. |
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| Product Citations | Azari S, et al. 2021. MBio. 12:e0184921. PubMed Burel JG, et al. 2019. Elife. 8. PubMed Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed |
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| RRID | AB_2721406 (BioLegend Cat. No. 367127) AB_2721407 (BioLegend Cat. No. 367128) |
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| Structure | GPI-linked membrane glycoprotein. |
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| Distribution | Monocytes, macrophages, dendritic cells, and granulocytes. |
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| Function | LPS receptor, clearance of Gram-negative pathogens. |
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| Interaction | LPS. |
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| Ligand/Receptor | LPS. |
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| Cell Type | Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Stocks SC, et al. 1990. Biochem. J. 268:275. Wright SD, et al. 1990. Science 4975:1431. |
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| Gene ID | 929 |
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| UniProt | View information about CD14 on UniProt.org |
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| Clone | 63D3 |
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| Regulatory Status | RUO |
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| Other Names | Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor |
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| Isotype | Mouse IgG1, κ |
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