Biolegend, 368404, Alexa Fluor® 647 anti-human Ikaros Antibody
100tests
Catalog Number: 368404
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. |
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| Product Citations | Ye Z, et al. 2021. Nat Commun. 12:907. PubMed |
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| RRID | AB_2566182 (BioLegend Cat. No. 368403) AB_2566183 (BioLegend Cat. No. 368404) |
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| Structure | Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD |
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| Distribution | Fetal and adult hematopoietic cells. |
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| Function | Regulates lymphocyte differentiation. |
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| Interaction | DNA. |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930. 2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736. 3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230. |
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| Gene ID | 10320 |
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| UniProt | View information about Ikaros on UniProt.org |
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| Clone | 16B5C71 |
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| Regulatory Status | RUO |
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| Other Names | IK1, IKZF1 |
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| Isotype | Mouse IgG2a, κ |
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