Biolegend, 368535, Alexa Fluor® 488 anti-human CD45 Antibody
25tests
Catalog Number: 368535
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human PBMC |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | It was found that the HI30 clone and the 2D1 clone can cross block each other's binding. |
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| Application References(PubMed link indicates BioLegend citation) | Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109. |
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| Product Citations | Nagpal N, et al. 2020. Cell Stem Cell. 26(6):896-909. PubMed Xu C, et al. 2022. iScience. 25:105123. PubMed Zobel MJ, et al. 2020. J Immunother Cancer. 8:00. PubMed Manzano-Muñoz A, et al. 2021. Front Cell Dev Biol. 9:695225. PubMed |
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| RRID | AB_2721363 (BioLegend Cat. No. 368535) AB_2721364 (BioLegend Cat. No. 368536) |
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| Structure | Type I transmembrane protein, 180 - 240 kD (multiple isoforms) |
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| Distribution | Hematopoietic cells, except circulating erythrocytes or platelets. |
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| Function | Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation. |
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| Ligand/Receptor | Galectin-1, CD2, CD3, and CD4. |
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| Cell Type | B cells, Dendritic cells, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, Protein Kinases/Phosphatase, TCRs |
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| Antigen References | 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85. |
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| Gene ID | 5788 |
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| UniProt | View information about CD45 on UniProt.org |
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| Clone | 2D1 |
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| Regulatory Status | RUO |
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| Other Names | Leukocyte Common Antigen (LCA), T200 |
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| Isotype | Mouse IgG1, κ |
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