Biolegend, 369503, Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody
25tests
Catalog Number: 369503
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes. |
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| Product Citations | Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed |
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| RRID | AB_2571895 (BioLegend Cat. No. 369503) AB_2571896 (BioLegend Cat. No. 369504) |
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| Structure | ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology. |
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| Distribution | Cytoplasm and the nucleus. |
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| Function | ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates. |
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| Interaction | ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10. |
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| Cell Type | Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Synaptic Biology |
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| Molecular Family | Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase |
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| Antigen References | 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814. |
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| Gene ID | 5594 |
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| UniProt | View information about ERK1/2 on UniProt.org |
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| Clone | 6B8B69 |
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| Regulatory Status | RUO |
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| Other Names | P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK) |
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| Isotype | Mouse IgG2a, κ |
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