Biolegend, 371105, Alexa Fluor® 647 anti-human Aiolos Antibody
25tests
Catalog Number: 371105
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
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| Product Citations | Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed |
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| RRID | AB_2572171 (BioLegend Cat. No. 371105) AB_2572172 (BioLegend Cat. No. 371106) |
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| Structure | 58kD, zinc finger protein. |
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| Distribution | T and B lymphocytes. |
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| Function | Hematopoetic-specific transcriptional activator and chromatin remodeler. |
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| Interaction | Forms heterodimers with Ikaros family members IKZF4 and IKZF5. |
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| Cell Type | B cells, T cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319. 2. Ma S, et al. 2008. Blood. 111:1396. 3. Billot K, et al. 2010. Leuk. Res. 34:289. 4. Morgan B, et al. 2004. EMBO J. 16:2004. 5. Wang JH, et al. 1998. Immunity. 9:543-53. 6. Hosokawa Y, et al. 1999. Genomics. 61:326. |
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| Gene ID | 22806 |
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| UniProt | View information about Aiolos on UniProt.org |
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| Clone | 16D9C97 |
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| Regulatory Status | RUO |
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| Other Names | IKZF3, AIO, ZNFN/A3 |
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| Isotype | Mouse IgG1, κ |
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