Biolegend, 371106, Alexa Fluor® 647 anti-human Aiolos Antibody
100tests
Catalog Number: 371106
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
|---|
| Product Citations | Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed |
|---|
| RRID | AB_2572171 (BioLegend Cat. No. 371105) AB_2572172 (BioLegend Cat. No. 371106) |
|---|
| Structure | 58kD, zinc finger protein. |
|---|
| Distribution | T and B lymphocytes. |
|---|
| Function | Hematopoetic-specific transcriptional activator and chromatin remodeler. |
|---|
| Interaction | Forms heterodimers with Ikaros family members IKZF4 and IKZF5. |
|---|
| Cell Type | B cells, T cells |
|---|
| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors |
|---|
| Molecular Family | Nuclear Markers |
|---|
| Antigen References | 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319. 2. Ma S, et al. 2008. Blood. 111:1396. 3. Billot K, et al. 2010. Leuk. Res. 34:289. 4. Morgan B, et al. 2004. EMBO J. 16:2004. 5. Wang JH, et al. 1998. Immunity. 9:543-53. 6. Hosokawa Y, et al. 1999. Genomics. 61:326. |
|---|
| Gene ID | 22806 |
|---|
| UniProt | View information about Aiolos on UniProt.org |
|---|
| Clone | 16D9C97 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | IKZF3, AIO, ZNFN/A3 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
