Biolegend, 373404, Alexa Fluor® 488 anti-human CD18 Antibody
100tests
Catalog Number: 373404
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human peripheral blood lymphocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes. |
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| Application References(PubMed link indicates BioLegend citation) | Singer II, et al. 1993. J. Immunol. 150:2844.Seth R, et al. 1991. FEBS Lett. 282:193. |
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| RRID | AB_2716019 (BioLegend Cat. No. 373403) AB_2716020 (BioLegend Cat. No. 373404) |
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| Structure | Integrin, type I transmembrane glycoprotein |
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| Distribution | Leukocytes |
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| Function | Adhesion and signaling in hematopoietic cells |
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| Interaction | Associates with CD11a, CD11b, or CD11c |
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| Ligand/Receptor | ICAMs |
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| Cell Type | Leukocytes, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Signal Transduction |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Hogg N, et al. 1999. J. Clin. Invest. 103:97. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Springer TA. 1994. Cell 76:301. |
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| Gene ID | 3689 |
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| UniProt | View information about CD18 on UniProt.org |
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| Clone | 1B4/CD18 |
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| Regulatory Status | RUO |
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| Other Names | Integrin beta-2, integrin beta chain, beta 2 |
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| Isotype | Mouse IgG2a, κ |
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