Biolegend, 375124, Alexa Fluor® 488 anti-human CD159a (NKG2A) Antibody
100tests
Catalog Number: 375124
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human NKG2A/CD94-transfectants |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Clone S19004C does not cross-react with mouse CD159a. |
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| RRID | AB_2922591 (BioLegend Cat. No. 375123) AB_2922591 (BioLegend Cat. No. 375124) |
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| Structure | Type II transmembrane proteins of the C-type lectin superfamily |
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| Distribution | NK cells, NKT cells, T cell subsets |
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| Function | Inhibiting NK cell receptor |
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| Ligand/Receptor | HLA-E |
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| Cell Type | Lymphocytes, NK cells, NKT cells, T cells |
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| Biology Area | Immuno-Oncology, Immunology |
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| Molecular Family | Immune Checkpoint Receptors |
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| Antigen References | Brooks AG, et al. 1999. J Immunol. 162:305-13. Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701. Braud VM, et al. 2003. Trends Immunol. 24:162-4. Kamiya T, et al. 2019. J Clin Invest. 129:2094. André P, et al. 2018. Cell. 175:1731. |
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| Gene ID | 3821 |
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| UniProt | View information about CD159a on UniProt.org |
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| Clone | S19004C |
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| Regulatory Status | RUO |
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| Other Names | Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A |
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| Isotype | Mouse IgG1, κ |
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