Biolegend, 375521, Alexa Fluor® 647 anti-human CD20 Antibody
25tests
Catalog Number: 375521
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CD20 transfectant |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| RRID | AB_3068250 (BioLegend Cat. No. 375521) AB_3068250 (BioLegend Cat. No. 375522) |
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| Structure | Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD |
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| Distribution | B cell, T cell subsets |
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| Function | B cell activation |
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| Interaction | The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). |
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| Ligand/Receptor | Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82 |
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| Cell Type | B cells, T cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Hultin LE, et al. 1993. Cytometry 14:196. Tedder T and Engel P. 1994. Immunol. Today 15:450. |
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| Gene ID | 931 |
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| UniProt | View information about CD20 on UniProt.org |
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| Clone | S18015E |
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| Regulatory Status | RUO |
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| Other Names | B1, Bp35 |
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| Isotype | Mouse IgG2a, κ |
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