Biolegend, 381505, Alexa Fluor® 488 anti-Puromycin Antibody
25tests
Catalog Number: 381505
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| Verified Reactivity | Human, Mouse, Rat, All species |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Puromycin from Streptomyces alboniger coupled to KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase. For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody. |
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| RRID | AB_2927825 (BioLegend Cat. No. 381505) AB_2927825 (BioLegend Cat. No. 381506) |
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| Distribution | Cytoplasm, Ribosomes |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Cell Biology, Protein Synthesis |
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| Antigen References | Schmidt E, et al. 2009. Nat Methods. 6:275-7. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075. |
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| Gene ID | NA |
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| UniProt | View information about Puromycin on UniProt.org |
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| Clone | 2A4 |
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| Regulatory Status | RUO |
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| Other Names | puromycin from streptomyces alboniger |
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| Isotype | Mouse IgG2a, κ |
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