Biolegend, 381506, Alexa Fluor® 488 anti-Puromycin Antibody
100tests
Catalog Number: 381506
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human, Mouse, Rat, All species |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Puromycin from Streptomyces alboniger coupled to KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase. For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody. |
|---|
| RRID | AB_2927825 (BioLegend Cat. No. 381505) AB_2927825 (BioLegend Cat. No. 381506) |
|---|
| Distribution | Cytoplasm, Ribosomes |
|---|
| Cell Type | Embryonic Stem Cells |
|---|
| Biology Area | Cell Biology, Protein Synthesis |
|---|
| Antigen References | Schmidt E, et al. 2009. Nat Methods. 6:275-7. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075. |
|---|
| Gene ID | NA |
|---|
| UniProt | View information about Puromycin on UniProt.org |
|---|
| Clone | 2A4 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | puromycin from streptomyces alboniger |
|---|
| Isotype | Mouse IgG2a, κ |
|---|
