Biolegend, 386410, Alexa Fluor® 647 anti-human Glypican-4 Antibody
100tests
Catalog Number: 386410
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | AA 23-529 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool). |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Qualiy tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | This antibody blocks Glypican-4 mediated Wnt3a signaling in vitro at concentrations from 0.1 - 5.0 µg/mL. |
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| RRID | AB_3720723 (BioLegend Cat. No. 386409) AB_3720723 (BioLegend Cat. No. 386410) |
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| Structure | Cell surface heparan sulfate proteoglycan |
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| Distribution | Cell membrane and nucleoplasm |
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| Function | Enhances Wnt signaling by acting as R-spondin co-receptor |
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| Interaction | PICK1, Insulin Receptor, Wnt3a, FGF2 |
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| Ligand/Receptor | Wnt3a |
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| Cell Type | Neurons |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Signal Transduction |
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| Molecular Family | APP/Aβ Degradation |
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| Antigen References | Sakane H, et al. 2012. J Cell Sci. 125:449-60. Hagihara K, et al. 2000. Developmental Dynamics. 219:353-67. Watanabe K, et al. 1995. J Cell Biol. 130:1207-18. Ussar, S. et al. 2012. Diabetes. 61:2289-98. |
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| Gene ID | 2239 |
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| UniProt | View information about Glypican-4 on UniProt.org |
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| Clone | A21050B |
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| Regulatory Status | RUO |
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| Other Names | GPC4 |
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| Isotype | Mouse IgG2b, κ |
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