Biolegend, 392115, Alexa Fluor® 647 anti-human P2RY12 Antibody
25tests
Catalog Number: 392115
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human P2RY12-transfected cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research. |
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| RRID | AB_3068171 (BioLegend Cat. No. 392115) AB_3068171 (BioLegend Cat. No. 392116) |
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| Distribution | Platelets, microglia |
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| Function | Inhibit the adenylyl cyclase second messenger system |
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| Ligand/Receptor | ADP, ATP |
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| Cell Type | Microglia, Platelets |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | GPCR, Purinergic Receptors |
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| Antigen References | 1. Lecchi A, et al. 2015. Blood. 125:1006. 2. Zhang K, et al. 2014. Nature. 509:115. 3. Zhang J, et al. 2014. Nature. 509:119. 4. Cornelissen I, et al. 2010. Proc. Natl. Acad. Sci. USA. 107:18605. 5. Paruchuri S, et al. 2009. J. Exp. Med. 206:2543. |
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| Gene ID | 64805 |
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| UniProt | View information about P2RY12 on UniProt.org |
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| Clone | S16001E |
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| Regulatory Status | RUO |
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| Other Names | ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled |
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| Isotype | Mouse IgG2a, κ |
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