Biolegend, 393203, Alexa Fluor® 488 anti-human CD27 Recombinant Antibody
25tests
Catalog Number: 393203
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| Verified Reactivity | Human |
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| Antibody Type | Recombinant |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | Clone QA17A18 does not block clones 0323, M-T271, or LG.3A10, indicating a unique epitope. |
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| Product Citations | Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed |
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| RRID | AB_2750088 (BioLegend Cat. No. 393203) AB_2750089 (BioLegend Cat. No. 393204) |
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| Structure | TNF-R superfamily, type I transmembrane glycoprotein. |
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| Distribution | Medullary thymocytes, T and B cell subsets, NK cells. |
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| Ligand/Receptor | CD70 |
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| Cell Type | B cells, NK cells, T cells, Thymocytes |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules, TCRs |
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| Antigen References | Knapp W, et al. 1989. Immunol. Today 10:253-8 Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. Hintzen R, et al. 1994. Immunol. Today 15:307. Agematsu K, et al. 1995. J. Immunol. 154:3627. |
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| Gene ID | 939 |
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| UniProt | View information about CD27 on UniProt.org |
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| Clone | QA17A18 |
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| Regulatory Status | RUO |
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| Other Names | S152, T14, TNFRSF7 |
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| Isotype | Mouse IgG1, κ |
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