Biolegend, 393406, Alexa Fluor® 647 anti-human CD45 Recombinant Antibody
100tests
Catalog Number: 393406
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| Verified Reactivity | Human |
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| Antibody Type | Recombinant |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Red Laser (633 nm) |
| Application Notes | Clone QA17A19 does not block clones 2D1 and HI30 indicating a unique epitope. |
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| Product Citations | Loh L, et al. 2024. Cell Rep. 114705:43. PubMed |
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| RRID | AB_2750082 (BioLegend Cat. No. 393405) AB_2750083 (BioLegend Cat. No. 393406) |
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| Structure | Type I transmembrane protein, 180 - 240 kD (multiple isoforms) |
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| Distribution | Hematopoietic cells, except circulating erythrocytes or platelets. |
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| Function | Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation. |
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| Ligand/Receptor | Galectin-1, CD2, CD3, and CD4. |
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| Cell Type | B cells, Dendritic cells, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, TCRs |
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| Antigen References | Thomas M. 1989. Annu. Rev. Immunol. 7:339. Trowbridge I, Thomas ML. 1994. Annu. Rev. Immunol. 12:85. |
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| Gene ID | 5788 |
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| UniProt | View information about CD45 on UniProt.org |
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| Clone | QA17A19 |
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| Regulatory Status | RUO |
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| Other Names | Leukocyte Common Antigen, LCA, T200 |
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| Isotype | Mouse IgG1, κ |
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