Biolegend, 393914, Alexa Fluor® 647 anti-human CD133 Antibody
100tests
Catalog Number: 393914
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CD133 transfectants |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | In-house testing suggests that clone S16015F does not block clones 293C3, AC133, and clone 7 that are also raised against human CD133. |
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| RRID | AB_3662291 (BioLegend Cat. No. 393913) AB_3662291 (BioLegend Cat. No. 393914) |
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| Structure | 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains |
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| Distribution | Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells |
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| Function | May play a role in cell differentiation, proliferation, and apoptosis |
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| Interaction | NAT8; NAT8B; CDHR1 |
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| Cell Type | Hematopoietic stem and progenitors |
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| Biology Area | Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | Yin AH, et al. 1997. Blood. 90:5002. Miraglia S, et al. 1997. Blood. 90:5013. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25. |
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| Gene ID | 8842 |
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| UniProt | View information about CD133 on UniProt.org |
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| Clone | S16015F |
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| Regulatory Status | RUO |
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| Other Names | Prominin-1, PROM1, AC133 |
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| Isotype | Mouse IgG2a, κ |
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