Biolegend, 394208, Alexa Fluor® 647 anti-human IL12RB2 Antibody
100tests
Catalog Number: 394208
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human IL12RB2 transfected cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Product Citations | Preechanukul A, et al. 2023. iScience. 107234:26. PubMed |
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| RRID | AB_2894595 (BioLegend Cat. No. 394207) AB_2894595 (BioLegend Cat. No. 394208) |
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| Structure | Type I transmembrane protein, 3 isoforms, foms an heterodimer with IL12RB1 |
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| Distribution | Activated T cells, TH1 cells, NK cells, B cells, plasma cells |
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| Function | Role in TH1 differentiation |
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| Ligand/Receptor | IL-12 |
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| Cell Type | B cells, NK cells, Plasma cells, T cells, Th1 |
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| Biology Area | Immunology |
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| Molecular Family | Cytokine/Chemokine Receptors |
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| Antigen References | LaMere SA, et al. 2017. J Immunol. 199:3158 Prigione I, et al. 2016. Immunobiology. 221:291 de Paus RA, et al. 2013. Mol Immunol. 56:380 Airoldi I, et al. 2008. Blood. 112:750 |
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| Gene ID | 3595 |
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| UniProt | View information about IL12RB2 on UniProt.org |
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| Clone | S16020B |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-12 receptor subunit beta-2 |
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| Isotype | Mouse IgG2a, κ |
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