Biolegend, 395905, APC anti-human IRF3 Antibody
25tests
Catalog Number: 395905
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human IRF-3 protein (entire protein) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) |
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| Rustagi A, et al. 2012. Methods. 59(2):225-32 |
| RRID | AB_2888751 (BioLegend Cat. No. 395905) AB_2888751 (BioLegend Cat. No. 395906) |
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| Structure | Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD. |
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| Distribution | Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes. |
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| Function | CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε. |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776 Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654 Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12 |
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| Gene ID | 3661 |
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| UniProt | View information about IRF3 on UniProt.org |
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| Clone | AR1 |
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| Regulatory Status | RUO |
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| Other Names | IRF3, IRF-3, interferon regulatory factor 3 |
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| Isotype | Mouse IgG1, κ |
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