Biolegend, 396423, Alexa Fluor® 488 anti-human/mouse Granzyme B Recombinant Antibody
25tests
Catalog Number: 396423
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human, Mouse |
|---|---|
| Antibody Type | Recombinant |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). |
|---|
| RRID | AB_2924601 (BioLegend Cat. No. 396423) AB_2924601 (BioLegend Cat. No. 396424) |
|---|
| Structure | 32 kD serine protease |
|---|
| Distribution | Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells |
|---|
| Function | Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46. |
|---|
| Interaction | Caspase-3 |
|---|
| Ligand/Receptor | Mannose-6-phosphate receptor |
|---|
| Cell Type | Neutrophils, T cells |
|---|
| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
|---|
| Molecular Family | Enzymes and Regulators, Proteases |
|---|
| Antigen References | Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761. |
|---|
| Gene ID | 300214939 |
|---|
| UniProt | View information about Granzyme B on UniProt.org |
|---|
| Clone | QA18A28 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase |
|---|
| Isotype | Rat IgG1, κ |
|---|
