Biolegend, 396424, Alexa Fluor® 488 anti-human/mouse Granzyme B Recombinant Antibody
100tests
Catalog Number: 396424
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Recombinant |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). |
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| RRID | AB_2924601 (BioLegend Cat. No. 396423) AB_2924601 (BioLegend Cat. No. 396424) |
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| Structure | 32 kD serine protease |
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| Distribution | Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells |
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| Function | Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46. |
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| Interaction | Caspase-3 |
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| Ligand/Receptor | Mannose-6-phosphate receptor |
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| Cell Type | Neutrophils, T cells |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
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| Molecular Family | Enzymes and Regulators, Proteases |
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| Antigen References | Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761. |
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| Gene ID | 300214939 |
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| UniProt | View information about Granzyme B on UniProt.org |
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| Clone | QA18A28 |
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| Regulatory Status | RUO |
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| Other Names | Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase |
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| Isotype | Rat IgG1, κ |
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