Biolegend, 397405, Alexa Fluor® 488 anti-human EOS Antibody
25tests
Catalog Number: 397405
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Human EOS recombinant protein (271-520 a.a.) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Clones W16032A and W16032B cross-block each other and likely recognize different epitopes. |
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| RRID | AB_3716911 (BioLegend Cat. No. 397405) AB_3716911 (BioLegend Cat. No. 397406) |
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| Structure | IKAROS family member |
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| Distribution | Lymphocytes, specifically in Tregs |
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| Function | Essential for inhibitory function of Tregs |
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| Interaction | Binds to 5'GGGAATRCC-3' Ikaros-binding sequence |
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| Antigen References | Honma Y, et al. 1999. FEBS Lett. 447:76-80. Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54. Westman BJ, et al. 2004. Biochemistry. 43:13318-27. Pan F, et al. 2009. Science. 10.1126/science.1176077. |
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| Gene ID | 64375 |
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| UniProt | View information about EOS on UniProt.org |
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| Clone | W16032B |
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| Regulatory Status | RUO |
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| Other Names | Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782 |
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| Isotype | Rat IgG2a, κ |
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