Biolegend, 397407, TotalSeq™-B1566 anti-human EOS Antibody
10microg
Catalog Number: 397407
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Human EOS recombinant protein (271-520 a.a.) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ICPG - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 0.5 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
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| Application Notes | Clones W16032A and W16032B cross-block each other and likely recognize different epitopes. |
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| Additional Product Notes | TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq. The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Scientific Poster Library. |
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| Structure | IKAROS family member |
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| Distribution | Lymphocytes, specifically in Tregs |
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| Function | Essential for inhibitory function of Tregs |
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| Interaction | Binds to 5'GGGAATRCC-3' Ikaros-binding sequence |
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| Antigen References | Honma Y, et al. 1999. FEBS Lett. 447:76-80. Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54. Westman BJ, et al. 2004. Biochemistry. 43:13318-27. Pan F, et al. 2009. Science. 10.1126/science.1176077. |
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| Gene ID | 64375 |
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| UniProt | View information about EOS on UniProt.org |
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| Clone | W16032B |
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| Regulatory Status | RUO |
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| Other Names | Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782 |
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| Isotype | Rat IgG2a, κ |
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| Barcode Sequence | CGAATTAACAGGTTC |
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