Biolegend, 398306, Alexa Fluor® 647 anti-human CD32B/C Antibody
100tests
Catalog Number: 398306
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A. |
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| RRID | AB_2860998 (BioLegend Cat. No. 398305) AB_2860999 (BioLegend Cat. No. 398306) |
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| Structure | Type I transmembrane protein, 40 kDa, member of the Ig superfamily |
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| Distribution | B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells. |
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| Function | Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation |
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| Ligand/Receptor | Fc region of complex or aggregated immunoglobulins gamma |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Monocytes, Platelets |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | Bruhns P, et al. 2009. Blood. 113: 3716. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735. Descours B, et al. 2017. Nature. 543: 564. Tomiyama Y, et al. 1992. Blood. 80: 2261. Indik Z, et al. 1991. J Clin Invest. 88: 1766. Ramsland PA, et al. 2011. J Immunol. 187: 3208. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311. Bournazos S, et al. 2009. J Immunol. 182: 8026. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437. Sandilands GP, et al. 1997. Immunology. 91: 204. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878. Gillis C, et al. 2014. Front Immunol. 5: 254. |
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| Gene ID | 22139103 |
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| UniProt | View information about FCGR2B+OR+FCGR2B on UniProt.org |
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| Clone | S18005H |
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| Regulatory Status | RUO |
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| Other Names | FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32B, CD32C, FCG2, FCGR2, IGFR2, FcRII-c, CDW32 |
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| Isotype | Mouse IgG1, κ |
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