| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human CD64-Fc (1257-FC-050) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. |
Answer:APC/Fire™ 750 Anti-Human CD64 Antibody, 399620.
Answer:Human CD64; APC/Fire 750; Flow cytometry.
Answer:APC/Fire™ 750 anti-human CD64 is a fluorophore-conjugated antibody for flow-cytometric characterization of human cells expressing CD64, also known as FcγRI.
APC/Fire™ 750 anti-human CD64 is a fluorophore-conjugated antibody for flow-cytometric characterization of human cells expressing CD64, also known as FcγRI. CD64 is a high-affinity Fc receptor commonly associated with monocytes, macrophages, and selected activated myeloid populations. The APC/Fire 750 tandem dye provides a far-red emission channel and is useful in multicolor immune phenotyping panels built around a compatible red-laser optical configuration. The reagent can help resolve CD64-positive populations alongside additional lineage or activation markers. Titration, compensation, and sample preparation should be established for the instrument and panel. The catalog identifier is preserved without inferring vial size or clone from related variants.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB). |
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| RRID | AB_3097524 (BioLegend Cat. No. 399619) AB_3097524 (BioLegend Cat. No. 399620) |
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| Structure | Ig superfamily, type I glycoprotein, 72 kD |
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| Distribution | Monocytes, macrophages, dendritic cells, activated granulocytes |
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| Function | Phagocytosis, ADCC |
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| Ligand/Receptor | IgG receptor |
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| Cell Type | Dendritic cells, Granulocytes, Macrophages, Monocytes |
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| Biology Area | Immunology, Inhibitory Molecules |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | Hulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127. van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21. |
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| Gene ID | 2209 |
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| UniProt | View information about CD64 on UniProt.org |
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| Clone | S18012E |
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| Regulatory Status | RUO |
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| Other Names | FcγRI, FcR I |
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| Isotype | Mouse IgG1, κ |
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