Biolegend, 400417, Alexa Fluor® 488 Rat IgG1, κ Isotype Ctrl Antibody
100microg
Catalog Number: 400417
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| Antibody Type | Monoclonal |
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| Host Species | Rat |
| Immunogen | Trinitrophenol + KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). |
| Application References(PubMed link indicates BioLegend citation) | Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed |
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| Product Citations | Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Reynolds A, et al. 2015. J Immunol. 194:231. PubMed |
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| RRID | AB_389319 (BioLegend Cat. No. 400417) |
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| Gene ID | NA |
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| Clone | RTK2071 |
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| Regulatory Status | RUO |
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| Isotype | Rat IgG1, κ |
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