Biolegend, 400525, Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl Antibody
100microg
Catalog Number: 400525
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| Antibody Type | Monoclonal |
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| Host Species | Rat |
| Immunogen | Trinitrophenol + KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
| Application Notes | Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA). |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application References(PubMed link indicates BioLegend citation) | Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA) |
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| Product Citations | Ojeda-Fernández L, et al. 2016. PLoS One. 12: 1005935. PubMed Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed Mizutani T, et al. 2023. iScience. 26:106081. PubMed Evans JG, et al. 2023. Viruses. 15:. PubMed Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed Xie L, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:435. PubMed El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed Ruetsche D, et al. 2022. Cells. 11:. PubMed Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed Takizawa S, et al. 2022. Mol Med. 28:91. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Weigelin B, et al. 2021. Nat Commun. 12:5217. PubMed |
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| RRID | AB_2864283 (BioLegend Cat. No. 400525) |
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| Gene ID | NA |
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| Clone | RTK2758 |
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| Regulatory Status | RUO |
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| Isotype | Rat IgG2a, κ |
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