Biolegend, 400625, Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl Antibody
100microg
Catalog Number: 400625
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| Antibody Type | Monoclonal |
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| Host Species | Rat |
| Immunogen | Trinitrophenol + KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control. |
| Application References(PubMed link indicates BioLegend citation) | Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed |
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| Product Citations | Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed Li X, et al. 2023. J Transl Med. 21:121. PubMed Wheeler R, Gull E 2015. Nat Commun. 6: 8964. PubMed Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed Palao N, et al. 2022. Int J Biol Sci. 18:5873. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Bochenek MA, et al. 2018. Nat Biomed Eng. 2:810. PubMed Marko C, et al. 2013. Am J Pathol. 183:35. PubMed O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed |
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| RRID | AB_389321 (BioLegend Cat. No. 400625) |
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| Gene ID | NA |
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| Clone | RTK4530 |
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| Regulatory Status | RUO |
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| Isotype | Rat IgG2b, κ |
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