Biolegend, 400715, Alexa Fluor® 488 Rat IgG2c, κ Isotype Ctrl Antibody
25microg
Catalog Number: 400715
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Category
| Antibody Type | Monoclonal |
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| Host Species | Rat |
| Immunogen | Trinitrophenol + KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
| Application Notes | The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control. |
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| Application References(PubMed link indicates BioLegend citation) | Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed |
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| Product Citations | Sato T, et al. 2016. J Pathol. 152:239. PubMed |
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| RRID | AB_3097658 (BioLegend Cat. No. 400715) |
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| Gene ID | NA |
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| Clone | RTK4174 |
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| Regulatory Status | RUO |
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| Isotype | Rat IgG2c, κ |
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