Biolegend, 400923, Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl Antibody
100microg
Catalog Number: 400923
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| Antibody Type | Monoclonal |
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| Host Species | Armenian Hamster |
| Immunogen | Trinitrophenol + KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg). |
| Application References(PubMed link indicates BioLegend citation) | Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. Yu R, et al. 2006. Obesity 14:1353. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed |
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| Product Citations | Wang L, et al. 2013. J Immunol. 190:1319. PubMed Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed Koh HS, et al. 2021. Int J Mol Sci. 22:. PubMed Ogita T, et al. 2020. Frontiers in Immunology. 0.721527778. PubMed Hirota J, et al. 2012. J Allergy Clin Immunol. 129:1116. PubMed Cho KA, et al. 2018. Mol Med Rep. 17:6723. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Kolawole E, et al. 2016. J Immunol. 196: 1461 - 1470. PubMed Stephens HM, et al. 2022. Methods Mol Biol. 2478:727. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed |
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| RRID | AB_2814703 (BioLegend Cat. No. 400923) |
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| Gene ID | NA |
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| Clone | HTK888 |
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| Regulatory Status | RUO |
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| Isotype | Armenian Hamster IgG |
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