Biolegend, 402453, Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl Antibody
100microg
Catalog Number: 402453
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| Antibody Type | Monoclonal |
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| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Certificate of Analysis online tool to find the exact concentrations of your lots of product. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA) |
| Additional Product Notes | For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only |
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| Application References(PubMed link indicates BioLegend citation) | Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed |
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| RRID | AB_3720602 (BioLegend Cat. No. 402453) |
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| Gene ID | NA |
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| Clone | MOPC-21 |
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| Regulatory Status | RUO |
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| Isotype | Mouse IgG1, κ |
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