Biolegend, 405103, HRP Avidin

Name: HRP Avidin
Brand: Biolegend
SKU: 405103

1ml

Catalog Number: 405103

Pricing

Formulation	Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation	Avidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling	Upon receipt, the antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application	ELISA - Quality tested ELISPOT, IHC, WB - Reported in the literature, not verified in house
Recommended Usage	Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes	Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Ordering

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