Biolegend, 405173, PE/Fire™ 700 Streptavidin
25microg
Catalog Number: 405173
| Verified Reactivity | Human, Mouse, Rat, All Species |
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| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions. |
| Concentration | 0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application Notes |
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| Streptavidin-PE/Fire™ 700 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Fire™ 700 is 330 kD and Streptavidin alone is 52 kD. |
| Gene ID | NA |
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| UniProt | View information about Biotin on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Streptavidin, SAV, SAV, SAV-PE/Fire™ 700 |
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