Biolegend, 405229, Brilliant Violet 605™ Streptavidin
100microl
Catalog Number: 405229
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| Verified Reactivity | Human, Mouse, Rat, All Species |
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| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | Streptavidin was conjugated with Brilliant Violet 605™ under optimal conditions. |
| Concentration | 0.1 mg/ml (concentration relates to the Streptavidin only component of the conjugate) |
| Storage & Handling | The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICFC - Verified |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Recommended Usage |
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| Each lot of this Streptavidin-Brilliant Violet 605™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 605™ conjugation. For immunofluorescent staining, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | The average molecular weight of Streptavidin-Brilliant Violet 605™ is 340 kD and Streptavidin alone is 52 kD. |
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| Application References(PubMed link indicates BioLegend citation) | Altin JA, et al. 2014. PNAS. 111:2067. PubMed Perez-Quintero LA, et al. 2014. J Exp Med. 211:727. PubMed Cartwright EK, et al. 2014. J. Immunol. 192:4666. PubMed Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed Arlehamnn CL, et al. 2014. J Immunol. 193:2931. PubMed Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed |
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| Product Citations | Valletta S, et al. 2020. Nat Commun. 3.288194444. PubMed Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed Nagashima K, et al. 2022. J Immunol. 209:5. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Barisas DAG, et al. 2023. PLoS Biol. 21:e3001746. PubMed Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed Geng G, et al. 2021. Elife. 10: . PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed Cao Y, et al. 2023. Nature. 614:521. PubMed Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed Friedman D, et al. 2022. J Immunol. 208:1845. PubMed Pascual G, et al. 2016. Nature. 541:41-45. PubMed Mehedi M, et al. 2016. PLoS Pathog. 12:e1006062. PubMed Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed Pérez-Quintero L, et al. 2014. J Exp Med. 211:727. PubMed Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed Arlehamn C, et al. 2014. J Immunol. 193:2931. PubMed Cartwright E, et al. 2014. J Immunol. 192:4666. PubMed Han YH, et al. 2021. Science. 373:. PubMed Cao Y, et al. 2022. Nature. :. PubMed Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed Lainé A, et al. 2015. J Immunol. 195: 1791-1803. PubMed Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed Hutter K, et al. 2020. FEBS J. . PubMed Chen G, et al. 2019. Curr Protoc Cell Biol. 82:e65. PubMed Ko KH, et al. 2018. Sci Rep. 8:16905. PubMed Schmid M, et al. 2016. PLoS One. 12: 1005676. PubMed Pineault KM, et al. 2019. Nat Commun. 10:3168. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed Delacher M, et al. 2020. Immunity. 52:295. PubMed Pascual G, et al. 2021. Nature. 599:485. PubMed Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed Liu R, et al. 2021. Mol Cell Biol. 41:e0025121. PubMed Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed Ma C, et al. 2016. J Exp Med. 213: 1589 - 1608. PubMed Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Altin J, et al. 2014. Proc Natl Acad Sci U S A. 111:2067. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Wells DK, et al. 2020. Cell. 183:818. PubMed Kolan S, et al. 2015. PLoS One. 10: 0134113. PubMed |
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| Gene ID | NA |
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| UniProt | View information about Biotin on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | SAv-Brilliant Violet 605™ |
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