Biolegend, 405319, Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody
100microg
Catalog Number: 405319
| Verified Reactivity | Mouse |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA. |
| Application References(PubMed link indicates BioLegend citation) | Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70. |
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| Product Citations | Frasca D, et al. 2020. Front Immunol. 11:392. PubMed Moore ER, et al. 2021. Bone. 143:115738. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed Rahimipour M, et al. 2021. Cell J. 23:154. PubMed Chen BR, et al. 2022. Methods Mol Biol. 2444:15. PubMed Dhande IS, et al. 2020. J Am Heart Assoc. 9:e014142. PubMed Varea O, et al. 2022. Brain Commun. 4:fcac168. PubMed Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed Trapecar M, et al. 2021. Sci Adv. 7:00. PubMed Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed Tomić S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.08.040. PubMed Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed Duran J, et al. 2020. Mol Neurobiol. 57:4657. PubMed Bianchi E, et al. 2021. Nat Commun. 12:1251. PubMed Slabicki M, et al. 2020. Nature. 588:164. PubMed Kim J, et al. 2022. Sci Rep. 12:7753. PubMed Wu H, et al. 2016. Sci Rep. 6:28065. PubMed Trapecar M, et al. 2020. Cell Systems. 10(3):223-239. PubMed Fu W, et al. 2021. Front Immunol. 12:656090. PubMed Zelenova EA, et al. 2021. Cells. 10:. PubMed Varea O, et al. 2021. Neurobiol Dis. 147:105173. PubMed Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed Wang X, et al. 2020. Immunity. 328:52. PubMed Nie Y, et al. 2021. Virol Sin. 36:692. PubMed Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed |
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| RRID | AB_2563044 (BioLegend Cat. No. 405319) |
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| Distribution | B cells |
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| Gene ID | 16059 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4053 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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