Biolegend, 405321, Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Antibody
25microg
Catalog Number: 405321
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| Verified Reactivity | Mouse |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA. |
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| Application References(PubMed link indicates BioLegend citation) | Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70. |
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| Product Citations | Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed Della Marina A, et al. 2021. Cells. 10:. PubMed Wong JJ, et al. 2021. Sci Adv. 7:. PubMed özdilek A, et al. 2020. Proc Natl Acad Sci U S A. 117:1280. PubMed Jung WH, et al. 2019. Cells. 8:813. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Middleton DR, et al. 2021. J Bacteriol. 203:. PubMed Chen BR, et al. 2021. Elife. 10:. PubMed Pruett L, et al. 2020. Laryngoscope. 130:2432. PubMed Bose S, et al. 2020. Nat Biomed Eng. 4:814. PubMed Khan AA, et al. 2022. Bioconjug Chem. . PubMed Zheng W, et al. 2020. Nature. 577:543. PubMed Smith R, et al. 2017. Biophys J. 10.1016/j.bpj.2017.05.005. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Pandey N, et al. 2020. J Cell Biol. 219:00:00. PubMed Scott WA, et al. 2021. PLoS Genet. 17:e1009909. PubMed |
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| RRID | AB_2563045 (BioLegend Cat. No. 405322) |
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| Distribution | B cells |
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| Gene ID | 16059 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4053 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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