Biolegend, 405326, Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody
100microg
Catalog Number: 405326
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Category
| Verified Reactivity | Mouse |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested IHC-F, IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application Notes | This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA. |
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| Application References(PubMed link indicates BioLegend citation) | Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70. |
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| Product Citations | Ambade AS, et al. 2019. Transl Res. 203:15. PubMed Farrell K, et al. 2022. Cell Rep. 41:111470. PubMed Aljobaily N, et al. 2022. Nutrients. 15: . PubMed Gurusamy M, et al. 2023. Eur J Med Res. 28:71. PubMed Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.119444444. PubMed Tada T, et al. 2022. Cell Rep. 38:110237. PubMed Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.105555556. PubMed Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed Tada T, et al. 2020. Cell Rep. 33:108528. PubMed Wagner W, et al. 2019. Cell Rep. 28:11. PubMed Antonyshyn JA, et al. 2021. Commun Biol. 4:1205. PubMed |
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| RRID | AB_2563308 (BioLegend Cat. No. 405326) |
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| Distribution | B cells |
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| Gene ID | 16059 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4053 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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