Biolegend, 405418, Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity) Antibody
100microg
Catalog Number: 405418
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| Verified Reactivity | Rat |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies. |
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| Application References(PubMed link indicates BioLegend citation) | Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P) |
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| Product Citations | Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed Wang JX, et al. 2023. JCI Insight. 8:. PubMed Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed Fowler FC, et al. 2022. Elife. 11: . PubMed Jeon EY, et al. 2023. Bioeng Transl Med. 8:e10362. PubMed Song JW, et al. 2021. Theranostics. 11:8874. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Coleman LG, et al. 2018. Brain Behav Immun. 72:61. PubMed Garcia GL, et al. 2020. Am J Pathol. 543:190. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Lisk C, et al. 2020. Front Immunol. 1.329166667. PubMed Blackshear CP, et al. 2018. Tissue Eng Part C Methods. 24:566. PubMed Chen BR, et al. 2021. Elife. 10:. PubMed Desbien A, et al. 2016. J Immunol. 197:4351-4359. PubMed Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed Huang L, et al. 2016. Cell Death Dis. 7:e2301. PubMed Naito H, et al. 2019. Dev Cell. 48:151. PubMed |
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| RRID | AB_2563120 (BioLegend Cat. No. 405418) |
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| Gene ID | 295279 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4054 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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